Manufacturing of Lysolecithin with High Lysophosphatidic Acid Content and Its Efficacy for Skin

Lysophosphatidic acid, referred to as LPA, is one of the functional Lysophospholipids. It has been reported that LPA shows various physiological effects as a lipid mediator, therefore LPA is interesting compounds, and this study was conducted to reveal the novel synthesis method of LPA highly contained in the Lysolecithin by enzymatic process and its high physiological activities of LPA.

Firstly, we found a specific wild type of microorganism which produces a high amount of target enzymes extracellularly. We confirmed that the supernatant from culture broth of this microorganism includes enough titer of enzyme for the reaction without the purification. These enzymes effectively convert from lecithin into a modified lecithin highly containing of Phosphatidic acid, referred to as PA. We applied Phospholipase A to PA rich lecithin to hydrolyze it to obtain an LPA rich Lysolecithin. We found this multifunctional Lysolecithin contains Lysophosphatidylinositol which has not obtained by conventional enzymatic acyl hydrolysis.

Secondary, we evaluated functional Lysolecithin benefits to skin barrier using in-vitro methods and clinical test. The functional Lysolecithin induced increase of the various barrier related genes in normal human epidermal keratinocytes. Regarding the clinical test, the efficiency of a face-cream containing a functional Lysolecithin for the against of sensitive skin on human volunteers was tested. We observed two significant benefits to skin that the stratum corneum moisture content increased, and the trans epidermal water loss decreased compared to the baseline, and additionally the stinging-score decreased significantly. These results indicated that functional Lysolecithin promotes the improvement of sensitive skin through the regulation of epidermal barrier maturation.

In this presentation, we report on the research results leading to the development of multifunctional Lysolecithin which is LPA and LPI rich contained and its physiological effects on the skin from our data in results of in-vitro, clinical test.