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Video

2025 AOCS Annual Meeting & Expo.

Industrial Oil Products

General Industrial Oil Products I

Supercritical Carbon Dioxide Extraction: Antioxidant Activity and Lipid Composition of Hemp seed oil in Thailand

Tuesday, April 29, 2025

11:15 AM – 11:35 AM PST

Room: C124

Presenting Author(s)

Teerasak Punvichai, PhD

Ph.D CHEMICAL ENGINEERING / LECTURER
Prince Of Songkla University
Muang Surat Thani, Surat Thani, Thailand

Co-Author(s)

JP

Jantarat Pipakdee

MS.C
PSU
Suratthani, Surat Thani, Thailand
HC

Hattipong Chaitham

MS.C STUDENT
PSU
Suratthani, Surat Thani, Thailand

Abstract: The factors affecting the quality of hemp seed extraction using supercritical carbon dioxide (SC-CO₂) techniques were examined in this study of the antioxidant and lipid compositions of hemp seed oil from Thailand. Fresh hemp seeds extracted at a temperature of 32 ºC, a pressure of 160 bar, and an extraction duration of 120 minutes had an extraction yield of 8.84±0.04%. The total phenolic content was highest at 154.2±4.6 mg GAE/g extract. The highest flavonoid content was 79.0±0.8 mg QAE/g extract. The antioxidant activity, assessed from DPPH radical scavenging and ABTS, showed the best results, with IC₅₀ values of 2.56±0.48 mg/mL and 0.7±0.0 mg/mL, respectively. The fresh hemp seed extract also had a significant CBD content of 0.022 mg/g. Additionally, the FRAP value of the extract from fresh hemp seeds was higher than that from dry seeds, measuring 141±3 and 128±2 mg FeSO₄/g extract, respectively. Fresh hemp seed extract is also a source of polyunsaturated fatty acids, constituting 72.91% of the content, with linoleic acid (C18:2n6c) having the highest 51.45% contribution.