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2025 AOCS Annual Meeting & Expo.

Lipid Oxidation and Quality

Health and Nutrition

Analytical

Application of Lipidomics in Lipid Oxidation and Quality

Optimized flaxseed oil influences intestinal lipid metabolism and reduces plasma oxidative stress

Monday, April 28, 2025
4:35 PM – 4:55 PM PST
Room: B116
Abstract:

Lipidomics provides critical insights into the relationship between lipid oxidation, intestinal health, and systemic oxidative stress. This study combines animal and human models to evaluate the potential of optimized flaxseed oil, enriched in natural antioxidants, in promoting intestinal metabolic health and mitigating oxidative stress.

In an animal model, the effects of fresh and stored conventional and optimized flaxseed oil on intestinal fatty acid oxidation (FAO) were investigated. Targeted LC-MS analysis revealed no significant changes in acylcarnitine levels in the jejunum. However, in the colon, fresh optimized flaxseed oil reduced total acylcarnitine content by ~50% compared to conventional oil, suggesting improved mitochondrial FAO efficiency and fewer pro-inflammatory lipid intermediates. Importantly, storage did not significantly alter acylcarnitine levels, highlighting the intrinsic oxidative stability of the optimized oils.

In a complementary human study, optimized flaxseed oil was shown to counteract exercise-induced oxidative stress. Targeted and untargeted lipidomics revealed significantly lower levels of oxidized phosphatidylcholines and oxysterols, including 7-ketocholesterol, in participants supplemented with the oil compared to controls. Increased plasma phospholipid content in the flaxseed oil group suggested reduced lipid oxidation susceptibility.

These findings highlight the dual role of optimized flaxseed oil in mitigating intestinal inflammation through improved FAO in animals and reducing systemic oxidative stress in humans. Lipidomics-enabled insights into lipid oxidation and metabolism underscore the potential of antioxidant-enriched oils to improve intestinal and systemic health, with implications for inflammatory conditions such as IBD and broader metabolic disorders.