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Video

2025 AOCS Annual Meeting & Expo.

Analytical

General Analytical Methods I

Rapid Preparation of Silver Thin-Layer Chromatography Plates in Less Than 10 Minutes

Monday, April 28, 2025

11:15 AM – 11:35 AM PST

Room: C123

Co-Author(s)

KN

Katherine A. Nelson

Associate Professor
University of Rochester Medical Center
Rochester, New York, United States

Presenting Author(s)

Andrew T. Nelson, MD, PhD

Assistant Professor
University of Rochester Medical Center
Rochester, NY, United States

Abstract: Silver ion thin-layer chromatography (Ag-TLC) is an invaluable tool in the lipid analyst’s armamentarium. Ag-TLC is uniquely useful in its ability to fraction fatty acid methyl esters (FAMEs) by the number and geometry of double bonds. Unfortunately, there are drawbacks to all three of the most common ways to access Ag-TLC plates: 1) commercially available Ag-TLC plates are expensive, costing over twice as much as their silver-less counterparts; 2) preparation of Ag-TLC plates from scratch is tedious, requires specialized equipment, and takes days; 3) conversion of commercial TLC plates to Ag-TLC plates is less expensive, but still a drawn out process, requiring hours to complete. Herein, we report a fast, operationally facile, inexpensive approach to transform commercial TLC plates into Ag-TLC plates. The process is complete in less than 10 minutes. These Ag-TLC plates readily resolved FAMEs from human serum and plasma by degree of unsaturation and stereochemistry.